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3D bioprinting of stem cells and analysis of microscopic images
Kandra, Mário ; Svoboda, Ondřej (referee) ; Kolářová, Jana (advisor)
In this diploma thesis we are discussing about using 3D bioprinting in tissue engineering. We are discribing using biomaterials for construction scaffolder and aplication stem cells in 3D bioprinting. Last section of theoretical part deals with very often used techniques of 3D bioprinting and we are focused on extrusion technique. In the practical part we propose a method for print vasculars structures. We realized prototype of print head, her design and 3D printing of individual parts. To mechanical part we create a control system for printing control. At the end we visualize the organization of the cells using program modules.
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Extruder head for 3D bioprinting of low viscosity hydrogels
Havlíček, Václav ; Harabiš, Vratislav (referee) ; Jaroš,, Josef (advisor)
In this bachelor thesis, I deal with 3D bioprinting for tissue engineering and analyze used materials and technologies that can be utilized for 3D bioprinting. The paper also includes a discussion of 3D bioprinting equipment and the current challenges of extrusion 3D bioprinting. In the practical part, a solution of a complex system for extrusion 3D bioprinting of low viscosity hydrogels is proposed using a hydraulic pump, a print head, and a cooling circuit.
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Upgrade of extruder head for 3D bioprinting of thermolabile hydrogels
Kedroň, Kryštof ; Harabiš, Vratislav (referee) ; Jaroš, Josef (advisor)
3D bioprinting is a perspective method of tissue engineering, capable of creating tissue structures with physiological function and spatial arrangement. The thesis deals with the use of this method for printing thermolabile hydrogels. The main goal of the thesis is to extend the print head for 3D printing of thermolabile hydrogels. The theoretical part is devoted to the description of 3D bioprinting, especially the important properties that each material must meet to be able to print, there is also a review of used hydrogels in bioprinting, focusing on thermolabile, print modalities, the possibility of cooling the printhead and a description of the ideal equipment for printing of thermolabile hydrogels. The practical part deals with the design and realization of the print head for 3D printing of thermolabile hydrogels, in the last part the achieved results are evaluated and discussed.
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Polymeric hydrogels preparation for tissue engineering applications
Pejskar, Michael ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
The thesis deals with polymer materials for preparation and bioprinting of hydrogels for tissue engineering. Theoretical part contains description of individual tissue types, description and research in the field of polymer materials for the preparation of hydrogels in tissue engineering and a description of 3D bioprinting. Practical part deals with the preparation, bioprinting, testing and analysis of hydrogels and their properties.
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Mechanisms of Vascularization in Skin Tissue Engineering
Futóová, Terézia ; Brož, Antonín (advisor) ; Šuca, Hubert (referee)
Tissue engineering is a multidisciplinary field dealing with the fabrication of artificial tissue substitutes for regenerative medicine. Current regenerative medicine uses various types of tissue grafts, which have different advantages and disadvantages depending on their origin, such as insufficient amount of replacement tissue when using autologous grafts or immunogenicity of allogeneic or xenogeneic grafts. An alternative could be artificial tissue replacement. Artificial tissue constructs may consist of a non-living matrix and a cellular component. The cellular component may remodel the construct, form a functional part of the construct, or help integrate the construct into the host body. A significant problem in the formation of such replacements is sufficient vascularization. It is essential to keep cells in larger tissue constructs alive. Vascularization can be enhanced by the addition of vascular endothelial cells that can form capillaries independently within the construct. Vascular formation can also be aided by angiogenic growth factors by their direct application to the construct or by their formation, e.g. in stem cells cultured in the construct. Another approach is 3D bioprinting, allowing direct placement of specific cell types, growth factors or biomaterials in the construct. This...
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Upgrade of extruder head for 3D bioprinting of thermolabile hydrogels
Kedroň, Kryštof ; Harabiš, Vratislav (referee) ; Jaroš, Josef (advisor)
3D bioprinting is a perspective method of tissue engineering, capable of creating tissue structures with physiological function and spatial arrangement. The thesis deals with the use of this method for printing thermolabile hydrogels. The main goal of the thesis is to extend the print head for 3D printing of thermolabile hydrogels. The theoretical part is devoted to the description of 3D bioprinting, especially the important properties that each material must meet to be able to print, there is also a review of used hydrogels in bioprinting, focusing on thermolabile, print modalities, the possibility of cooling the printhead and a description of the ideal equipment for printing of thermolabile hydrogels. The practical part deals with the design and realization of the print head for 3D printing of thermolabile hydrogels, in the last part the achieved results are evaluated and discussed.
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Extruder head for 3D bioprinting of low viscosity hydrogels
Havlíček, Václav ; Harabiš, Vratislav (referee) ; Jaroš,, Josef (advisor)
In this bachelor thesis, I deal with 3D bioprinting for tissue engineering and analyze used materials and technologies that can be utilized for 3D bioprinting. The paper also includes a discussion of 3D bioprinting equipment and the current challenges of extrusion 3D bioprinting. In the practical part, a solution of a complex system for extrusion 3D bioprinting of low viscosity hydrogels is proposed using a hydraulic pump, a print head, and a cooling circuit.
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3D bioprinting of stem cells and analysis of microscopic images
Kandra, Mário ; Svoboda, Ondřej (referee) ; Kolářová, Jana (advisor)
In this diploma thesis we are discussing about using 3D bioprinting in tissue engineering. We are discribing using biomaterials for construction scaffolder and aplication stem cells in 3D bioprinting. Last section of theoretical part deals with very often used techniques of 3D bioprinting and we are focused on extrusion technique. In the practical part we propose a method for print vasculars structures. We realized prototype of print head, her design and 3D printing of individual parts. To mechanical part we create a control system for printing control. At the end we visualize the organization of the cells using program modules.
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